Ramularia endophylla
Ramularia endophylla Verkley & U. Braun, in Verkley et al., Mycol. Res. 108(11): 1276 (2004).
≡ Sphaeria punctiformis Pers., Ann. Bot. (Usteri) 11: 26 (1794).
= Mycosphaerella punctiformis (Pers.) Starbäck, Bih. K. svenska VetenskAkad. Handl., Afd. 3 15(no. 2): 9 (1889).
Index Fungorum number: IF 500737; Facesoffungi number: FoF 11213, Fig. 1–3
Description: see Hyde et al. (2013); Videira et al. (2017).
Material examined: see Hyde et al. (2013); Videira et al. (2017).
Fig. 1 Mycosphaerella punctiformis (= Ramularia endophylla; re-illustrated from Hyde et al. 2013). a Label and herbarium specimens of Mycosphaerella punctiformis. b Appearance of ascomata on host surface. c Section through ascoma. d Section through peridium. e–h Ascus. i–l Ascospores. Scale bars: c = 50 μm, d–h = 10 μm, i–l =5 μm.
Fig. 2 Mycosphaerella punctiformis (epitype, CBS herb. 7949, re-drawn from Verkley et al. 2014). a Ascospores and asci in planta. b Germinating ascospores on MEA. Scale bars = 10 μm.
Fig. 3 Mycosphaerella punctiformis (CBS 113265 – ex-epitype). Conidiogenous cells and conidia in culture. Scale bar =10 μm.
Importance and distribution
Species of Mycosphaerella (= Ramularia) are plant pathogens, saprobes, endophytes (saprobic or plant-pathogenic), and some have mutualistic (in lichen) associations (Crous et al. 2000, 2001; Verkley et al. 2004). Many species of Mycosphaerella (= Ramularia) have been reported to cause severe leaf spot, defoliation and shoot die-back (Crous 1998; Crous et al. 2004b, 2006g; Hunter et al. 2006a, b; Burgess et al. 2007). There are 1588 Mycosphaerella epithets in Index Fungorum (2022), but many have been synonymized or transferred to other genera including Amycosphaerella, Arecophila, Austroafricana, Batcheloromyces, Cercospora, Cladosporium, Davidiella, Delphinella, Didymella, Fusoidiella, Keissleriella, Metasphaeria, Pallidocercospora, Parateratosphaeria, Passalora, Pezicula, Phyllachora, Planistromella, Sphaerellothecium, Stigmidium, Teratosphaeria and Venturia. There are 1,252 species as Mycosphaerella and 410 species as Ramularia known on a wide range of hosts and geographical locations. Mycosphaerella needs a thorough revision and an updated monograph of accepted species is required. No doubt many more species of Mycosphaerella will be discovered in the future but they need to be substantiated with DNA molecular data.
Industrial relevance and applications
Mycosphaerella is useful to the medical and pharmaceutical industry due to the ability to produce wide range of compounds such as (-) mycousnine which exhibits high and selective immunosuppressive activity on T Cells (Wang et al. 2017).
Quarantine significance
Mycosphaerella polygoni-cuspidati is a potential biological control agent for the invasive weed Fallopia japonica (Japanese knotweed), which is bothersome in Europe and North America (Kurose et al. 2016). Other species of Mycosphaerella may have potential to control other pathogens due to their ability to colonize dead tissue of a non-host, perhaps to produce propagules to enable onwards dispersal (“pogo stick hypothesis”) (Verkley et al. 2014).
Biochemical importance of the genus, chemical diversity or applications
Members of Mycosphaerella (= Ramularia) have the ability to produce a wide array of chemicals such as -(2-butyl)-6-ethyl-3-hydroxy-6-methylcyclohex-2-ene-1,5-dione, 3-(2-butyl)-6-ethyl-5-hydroxy-2-methoxy-6-methyl-cyclohex-2-enone (Moreno et al. 2011). Other compounds such as -Mycousunine and (±)-Isomycousunine which are phytotoxic usunic acid derivatives have been reported from M. nawae (Sassa et al. 1989). They may also produce various enzymes, phytotoxins (e.g. fijiensin) and secondary compounds (Upadhyay et al. 1990).
References
Arx JA von. 1983 – Mycosphaerella and its anamorphs. Proceedings van de Koninklijke Nederlandse Akademie van Wetenschappen Series 86, 15–54.
Crous PW, Verkley GJM, Groenewald JZ, Samson RA. 2009e – Fungal Biodiversity. CBS Laboratory Manual Series 1: 1–269. CBS-KNAW Fungal Biodiversity Centre, Utrecht, the Netherlands.
Johanson CJ. 1885 – Svampar frăn Island. Bestämda af C.J. Johanson. Öfversigt af Kongl. VetenskapsAkademiens Föhandlingar 41, 157–174.
Kirschner R. 2009 – Cercosporella and Ramularia. Mycologia 101, 110–119.
Klebahn H. 1918 – Borntraeger; Leipzig, Germany: Haupt und Nebenfruchtformen der Askomyzeten.
Laibach F. 1922 – Untersuchungen über einige Ramularia und Ovularia-Arten und ihre Beziehungen zur Ascomyzetengat tung Mycosphaerella. II. Centralblatt für Bakteriologie und Parasitenkunde, Zweite Abtheilung 55, 284–293.
Crous PW, Groenewald JZ. 2006 –Mycosphaerella alistairii. Fungal Planet No. 4.
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