Citation: Pem D et al. (2019) Mycosphere Notes 275-324: A morphotaxonomic revision and typification of obscure Dothideomycetes genera (incertae sedis). Mycosphere 10(1), 1115–1246
Bahusutrabeeja Subram. & Bhat, Can. J. Bot. 55(16): 204 (1977)
Saprobic on dead stems, twigs, leaves and branches. Sexual morph: undetermined. Asexual morph: Hyphomycetous. Mycelium hairy, medium dense, partly immersed, partly superficial, composed of thin, branched, septate, smooth-walled, light brown 2–3 µm hyphae. Setae and hyphopodia absent. Conidiophores distinct, macronematous, mononematous, solitary, erect, straight or slightly flexuous, septate, unbranched, rarely branched, cylindrical, smooth, thick-walled, dark reddish brown near the base, light brown in the apical part. Conidiogenous cells monophialidic, terminal, integrated, light brown, smooth, with a conspicuous flared, colarrette at the tip. Conidia solitary, simple, slimy, endogenous, hyaline, aseptate, smooth, thick-walled, rounded-cubical to polygonal, spherical to obovoid, hyaline to sub-hyaline, with flexuous, thin, hyaline appendages distributed all over the surface.
Type species – Bahusutrabeeja dwaya Subram. & Bhat
Notes – The genus Bahusutrabeeja was introduced by Subramanian & Bhat (1977) with Bahusutrabeeja dwaya Subraman. & Bhat as the type species. Bahusutrabeeja dwaya was isolated from dead twigs of Coffea arabica L. from India. Currently, five species are accommodated in the genus Bahusutrabeeja including B. angularis V.G. Rao & de Hoog, B. bunyensis McKenzie, B. dubhashii Bhat, B. exappendiculata Xiao X. Li & X.G. Zhang and B. globosa Bhat & W.B. Kendr.. Li et al. (2013) provided a key to species in the genus. The sexual morph is unknown. In a DNA based phylogenetic study conducted by Shenoy et al. (2010), the genus Bahusutrabeeja clustered in the Botryosphaeriales. It is currently placed in Botryosphaeriales, genera incertae sedis (Wijayawardene et al. 2018). However, in a study of Botryospheriales by Liu et al. (2012), Phillips et al. (2013) and Yang et al. (2016), there is no mention of the genus Bahusutrabeeja. We re-studied a specimen of B. dwaya and illustrated the characters (Fig. 13). We also conducted phylogenetic analyses using the putative strain of Bahusutrabeeja (GUFCC 4904). The LSU sequence from GUFCC 4904 corresponds to Neodeightonia palmicola. However, this strain is not related to the holotype and not supported by morphology. Species of Neodeightonia are characterized by spherical to globose, initially hyaline and pale to dark brown conidia with fine striations (Punithalingam 1969, Crous et al. 2006, Phillips et al. 2008, Phillips et al. 2013). However, in the genus Bahusutrabeeja, conidia are hyaline to sub-hyaline with appendages distributed all over the surface. Also, Bahusutrabeeja is a hyphomycetous genus, while species of Neodeightonia and Botryosphaeriaceae are coelomycetous. It is unlikely to place a hyphomycetous genus within the Neodeightonia clade. It seems that there must have been a mix-up of cultures and this isolate is not reliable. On the other hand, a BLAST search using the ITS sequence of CBS 261.77, the ex-type culture of Bahusutrabeeja dwaya shows 92 to 96 % similarity to the strains of Chaetosphaeriales sp. in the Sordariomycetes. We therefore transfer the genus Bahusutrabeeja to Chaetothyriales, family incertae sedis.
Crous PW, Schroers HJ, Groenewald JZ, Braun U, Schubert K. 2006 – Metulocladosporiella gen. nov. for the causal organism of Cladosporium speckle disease of banana. Mycological Research, 110, 264–275.
Li DW, Zhao G, Yang C, Jalsrai A, Kerin B. 2013 – Four noteworthy hyphomycetes from indoor environments. Mycotaxon 125, 111–121.
Liu JK, Phookamsak R, Doilom M, Wikee S, Li YM, et al. 2012 – Towards a natural classification of Botryosphaeriales. Fungal Diversity 57, 149–210.
Paulus BC. 2004 – The diversity and distribution of microfungi in leaf litter of an Australian wet tropics rainforest. PhD thesis James Cook University. Available at: https://researchonline.jcu.edu.au/1308/2/02whole.pdf (Accessed 9 April 2019).
Phillips AJL, Alves A, Abdollahzadeh J, Slippers B et al. 2013 – The Botryosphaeriaceae: genera and species known from culture. Studies in Mycology 76, 51–167.
Phillips AJL, Alves A, Pennycook SR, Johnston PR et al. 2008 – Resolving the phylogenetic and taxonomic status of dark-spored teleomorph genera in the Botryosphaeriaceae. Persoonia 21, 29–55.
Punithalingam E. 1969 – Studies on Sphaeropsidales in culture. I. Mycological Papers. 119, 1–24.
Shenoy BD, Jeewon R, Wang H, Amandeep K et al. 2010 – Sequence data reveals phylogenetic afﬁnities of fungal anamorphs Bahusutrabeeja, Diplococcium, Natarajania, Paliphora, Polyschema, Rattania and Spadicoides. Fungal Diversity 44, 161–169.
Subramanian CV, Bhat DJ. 1977 – Bahusutrabeeja, a new genus of the Hyphomycetes. Canadian Journal of Botany 55 (16), 2202–2206.
Wijayawardene NN, Hyde KD, Lumbsch HT, Liu JK et al. 2018 – Outline of Ascomycota: 2017. Fungal Diversity 88, 167–263.
Yang T, Groenewald JZ, Cheewangkoon R, Jami F et al. 2016 – Families, genera, and species of Botryosphaeriales. Fungal Biology 121, 322–346.